Discovery of protein‐protein interactions by affinity purification and mass spectrometry (AP‐MS)
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چکیده
Cells/tissues are then lysed and the Ab is added. Recovery of the Ab‐ t‐X complex is then done mostly using a capture resin made of ProteinA‐coated beads which bind the Fc portion of the Ab. However other systems exist which rely on other types of affinity interactions, i.e. biotinylation (strept)avidin systems or (His)6 Ni2+ columns etc. It is important to note that, several interactions (often non covalent ones) have to be conserved all along the purification procedure for the final complex to be recovered.
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